Journal: PeerJ

Article Title: PLA2G16 expression predicts prognosis and gemcitabine sensitivity in patients with pancreatic cancer.

doi: 10.7717/peerj.19517

Figure Lengend Snippet: Figure 2 The expression of PLA2G16 strongly correlates with the OS of pancreatic cancer patients. (A) Multivariate Cox analysis of PLA2G16 expression and other clinical pathological factors, including age, gender, and tumor grade, *: p < 0.05, ***: p < 0.001. (B) Correlation of overall survival rate with PLA2G16 expression in PAAD was analyzed by GEPIA. (C) Correlation of disease-free survival rate with PLA2G16 expression in PAAD was analyzed by GEPIA. (D) Overall survival curves for 102 patients treated with gemcitabine. Full-size

Article Snippet: BxPC-3 and PANC-1 cells within 5–10 passages after thawing to minimize genetic drift were treated with gemcitabine (Cat#HY-17026; Med Chem Express, Monmouth Junction, NJ, USA) at an initial concentration of 0.50 mM (1/4 IC50 for BXPC-3) or 0.49 mM (1/4 IC50 for PANC-1).

Techniques: Expressing

Journal: PeerJ

Article Title: PLA2G16 expression predicts prognosis and gemcitabine sensitivity in patients with pancreatic cancer.

doi: 10.7717/peerj.19517

Figure Lengend Snippet: Figure 3 PLA2G16 contributes to gemcitabine resistance in pancreatic cancer cells. (A) Differential expression analysis of PLA2G16 in SW1990 and SW1990/GR cells. Data retrieved from the GEO database. (B) Differential expression analysis of PLA2G16 in BxPC-3 and BxPC-3/GR cells. (C) Differential expression analysis of PLA2G16 in PANC-1 and PANC-1/GR cells. (D) Expression of PLA2G16 at protein level in BxPC-3 and PANC-1 drug-resistant cells. (E) IC50 of BxPC-3 and PANC-1 cells to gemcitabine. (F) IC50 of BxPC-3/GR and PANC-1/GR cells to gemcitabine.

Article Snippet: BxPC-3 and PANC-1 cells within 5–10 passages after thawing to minimize genetic drift were treated with gemcitabine (Cat#HY-17026; Med Chem Express, Monmouth Junction, NJ, USA) at an initial concentration of 0.50 mM (1/4 IC50 for BXPC-3) or 0.49 mM (1/4 IC50 for PANC-1).

Techniques: Quantitative Proteomics, Expressing

Journal: Nature Communications

Article Title: TRPC4/TRPC5 are critical for neuronal modulation by transcranial focused ultrasound in retrosplenial cortex in male mice

doi: 10.1038/s41467-026-74779-2

Figure Lengend Snippet: a Example diagram of the targeted recombination in the active population (TRAP) system design: bilateral microinjection of AAV9-Egr1-ER T2 CreER T2 -mCherry and a Cre-dependent AAV2/9-Syn-DIO-hM4Di-eGFP hybrid virus (Egr1TRAP) into RSC; tFUS activates Egr1 expression, resulting in subsequent expression of ER T2 CreER T2 -mCherry. Intraperitoneal injection of 4-hydroxytamoxifen (4-OHT) allows Cre to enter the nucleus and drive the expression of recombinant Cre-dependent hM4Di-eGFP. Created in BioRender. Wu, C. (2026) https://BioRender.com/vpmqvag . b Schematic diagram of the timeline for verifying the TRAP system’s efficiency. Three weeks after injecting a viral mixture of AAV9-Egr1-ERT2-Cre-ERT2-mCherry and AAV9-Syn-DIO-GFP, we administered an intraperitoneal injection of 4-OHT 30 min following tFUS. We then euthanized the mice three days later to verify the expression of cells captured by tFUS TRAP. Created in BioRender. Wu, C. (2026) https://BioRender.com/5ineha7 . c Quantification of tFUS TRAPed cells in Ctrl and tFUS groups. Two-sided unpaired t -test, t = 2.595, P = 0.0169, Ctrl: 12 slices from 8 mice, tFUS: 11 slices from 8 mice. d Confocal images of tFUS TRAPed cells in RSC. White arrowheads indicate tFUS TRAPed cells (cyan): overall scale bar, 200 μm. The magnified images correspond to the regions enclosed by the white dashed lines. Scale bar of the enlarged area, 20 μm. e Experimental design for the TRAP system with the behavioral study. Created in BioRender. Wu, C. (2026) https://BioRender.com/ea0ua8l . f The effect of the TRAP system inhibits tFUS-activated Egr1 + cells on PWT. Two-way RM ANOVA, interaction: F (2,42) = 7.01, P = 0.0024; n (TRAP-Ctrl) = 11 mice, n (TRAP) = 12 mice. g The effect of the TRAP system inhibits tFUS-activated Egr1 + cells on TWL. Two-way RM ANOVA, interaction: F (2, 42) = 3.16, P = 0.053; n (TRAP-Ctrl) = 11 mice, n (TRAP) = 12 mice. Source data are provided as a Source Data file. All data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001; ns not significant.

Article Snippet: Thirty minutes later, 4-hydroxytamoxifen (4-OHT, 25 mg/kg, i.p., Med Chem Express, Cat# HY-16950) was administered to induce nuclear translocation of Cre.

Techniques: Microinjection, Virus, Expressing, Injection, Recombinant

Journal: bioRxiv

Article Title: A circular engineered sortase for interrogating histone H3 in chromatin

doi: 10.1101/2024.09.10.612318

Figure Lengend Snippet: ( A ) Workflow for isolating tandem mass tagged histone H3 tails with cW11 sortase. ( B ) SDS-PAGE of 14-hour sortase reaction in a nuclear acid extract. ( C ) Tris-Tricine PAGE of histone H3(1-34) synthetic standards (1: 140 ng; 2: 420 ng) and TCA precipitated H3(1-32)-TMT from the acid extract reaction; bands between 25 and 37 kDa are histone H1. ( D ) Structure of the TMT-labelled oligoglycine peptide illustrating sortase reactive GGG, charge carrying K and carboxamide, and TMT labelled aminoalanine. ( E ) Unique proteoforms detected in each 6plex sample and shared between the two samples. ( F ) Quantifiable proteoforms detected in each 6plex sample and shared between the two samples. Volcano plot of significant (p<0.05) log2-fold change and overall abundance of H3 proteoforms in HEK293T cells following treatment with ( G ) LSD1/HDAC1/CoREST complex-specific inhibitor Corin, or ( H ) pan class one HDAC inhibitor MS275.

Article Snippet: For treatment with DMSO vehicle, MS275 (LC Laboratories, Cat# E-3866, Lot: ENT-102) or Corin (Med Chem Express, Cat# HY-111048/CS0034060, Lot: 41808), a single 15 cm plate of HEK293T cells was grown to near confluence and split.

Techniques: SDS Page